Parasitological analysis of sugarcane juice marketed in the Federal District

Authors

  • Fabiana Brandão
  • Priscila Fernandes da Mota
  • Lycia Batista Oliveira Monteiro
  • Lis Shadday
  • Caroline Araújo
  • Kellysson Gabriel B. Mendes
  • Aline Kelen Vesely Reis

Keywords:

Food Contamination, Cane juice, Parasitic diseases

Abstract

Objective:  to assess samples of sugarcane juice marketed in the Federal District in order to investigate the presence of parasites that can be harmful to health, causing Foodborne Diseases. Method: 10 samples of sugarcane juice from establishments distributed in different regions in the Federal District were analyzed. All samples were submitted to the Sedimentation method and analyzed in triplicate. The analysis was performed with the aid of optical microscopy to investigate the presence of eggs, cysts, oocysts or other parasitic evolutionary forms. Results: In all samples, the presence of some type of contamination was found, with yeasts being registered in 100% of the samples, in 50% Entamoeba sp., 10% Taenia sp., 10% Giardia sp. and in 10% Ascaris sp. Conclusion: We observed an inefficiency in the hygienic-sanitary practices of the establishments responsible for the processing and grinding of the vegetable, allowing contamination in most of the samples. This contamination can cause different types of disease for humans, particularly they offer more risks to immunocompromised individuals, requiring more effective monitoring of this type of food, as well as its distribution.

References

Kusumaningrum HD, van Asselt ED, Beumer RR, Zwietering MH. A quantitative analysis of cross-contamination of Salmonella and Campylobacter spp. via domestic kitchen surfaces. J Food Prot. 2004 Sep;67(9):1892–903.

Oliveira ACG, Seixas ASS, Sousa CP, Souza CWO. Microbiological evaluation of sugarcane juice sold at street stands and juice handling conditions in São Carlos, São Paulo, Brazil. Cad Saude Publica. 2006 May;22(5):1111–4.

de Souza Sant’Ana A. Quantitative Microbiology in Food Processing: Modeling the Microbial Ecology. John Wiley & Sons; 2016. 696 p.

Marine SC, Martin DA, Adalja A, Mathew S, Everts KL. Effect of market channel, farm scale, and years in production on mid-Atlantic vegetable producers’ knowledge and implementation of Good Agricultural Practices. Food Control. 2016 Jan 1;59:128–38.

Maldonade IR, Ginani VC, Riquette RFR, Gurgel-Gonçalves R, Mendes VS, Machado ER. Good manufacturing practices of minimally processed vegetables reduce contamination with pathogenic microorganisms. Rev Inst Med Trop Sao Paulo. 2019 Feb 14;61:e14.

Machado ER, Maldonade IR, Riquette RFR, Mendes VS, Gurgel-Gonçalves R, Ginani VC. Frequency of Enteroparasites and Bacteria in the Leafy Vegetables Sold in Brazilian Public Wholesale Markets. J Food Prot. 2018 Apr;81(4):542–8.

Hald T, Aspinall W, Devleesschauwer B, Cooke R, Corrigan T, Havelaar AH, et al. World Health Organization Estimates of the Relative Contributions of Food to the Burden of Disease Due to Selected Foodborne Hazards: A Structured Expert Elicitation. PLoS One. 2016 Jan 19;11(1):e0145839.

Meng X-J. From barnyard to food table: the omnipresence of hepatitis E virus and risk for zoonotic infection and food safety. Virus Res. 2011 Oct;161(1):23–30.

Both JMC. A desinfecção como barreira sanitária na prevenção de Doenças Transmitidas por Alimentos (DTA): sensibilidade de amostras de Staphylococcus aureus isoladas em alimentos no IPB-LACEN/RS, nos anos de 2002 a 2006, frente ao hipoclorito de sódio [Internet]. Vol. 36, Acta Scientiae Veterinariae. 2018. p. 77. Available from: http://dx.doi.org/10.22456/1679-9216.17258

Januskevicius V, Januskeviciene G, Zaborskiene G. Quality of beef diaphragm meat in naturally occurring Sarcocystic infection in cattle [Internet]. Vol. 36, Czech Journal of Food Sciences. 2018. p. 378–85. Available from: http://dx.doi.org/10.17221/89/2017-cjfs

Morais E de, de Morais E, de Carvalho C, de Carvalho C, Barbosa F, Pereira D. Controle Microbiológico com Relação às Doenças Transmitidas Por Alimentos: um Estudo de Revisão Literária [Internet]. XXI I Congresso Brasileiro de Nutrologia. 2018. Available from: http://dx.doi.org/10.1055/s-0038-1674569

World Health Organization. WHO estimates of the global burden of foodborne diseases. world health organization [internet]. 2015; available from: https://www.who.int/foodsafety/areas_work/foodborne-diseases/ferg/en/

Kirk MD, Pires SM, Black RE, Caipo M, Crump JA, Devleesschauwer B, et al. World Health Organization Estimates of the Global and Regional Disease Burden of 22 Foodborne Bacterial, Protozoal, and Viral Diseases, 2010: A Data Synthesis. PLoS Med. 2015 Dec;12(12):e1001921.

Schoeni JL, Wong ACL. Bacillus cereus food poisoning and its toxins. J Food Prot. 2005 Mar;68(3):636–48.

Granum PE, Lund T. Bacillus cereus and its food poisoning toxins. FEMS Microbiol Lett. 1997 Dec 15;157(2):223–8.

Hennekinne J-A, De Buyser M-L, Dragacci S. Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation. FEMS Microbiol Rev. 2012 Jul;36(4):815–36.

Dolan LC, Matulka RA, Burdock GA. Naturally occurring food toxins. Toxins . 2010 Sep;2(9):2289–332.

Odumeru JA. Microbial Safety of Food and Food Products [Internet]. Food Biochemistry and Food Processing. 2012. p. 785–97. Available from: http://dx.doi.org/10.1002/9781118308035.ch41

Gubernot DM, Lucey CT, Lee KC, Conley GB, Holness LG, Wise RP. Babesia Infection through Blood Transfusions: Reports Received by the US Food and Drug Administration, 1997–2007. Clin Infect Dis. 2009 Jan 1;48(1):25–30.

World Health Organization. Accelerating work to overcome the global impact of neglected tropical diseases : a roadmap for implementation : executive summary [Internet]. World Health Organization; 2012 [cited 2019 Sep 8]. Report No.: WHO/HTM/NTD/2012.1. Available from: https://www.who.int/iris/handle/10665/70809

Araújo EDAS, Santos JAP. O desenvolvimento da cultura da cana-de-açúcar no brasil e sua relevância na economia nacional. FACIDER - Revista Científica [Internet]. 2013 Jan 9 [cited 2019 Apr 17];4(4). Available from: http://sei-cesucol.edu.br/revista/index.php/facider/article/view/37

Sartori JA de S, de Souza Sartori JA. Qualidade físico-química da cana-de-açúcar e microbiológica do caldo sobre o processo de ozonização do caldo na redução sustentável da cor ICUMSA e impurezas vegetais [Internet]. Available from: http://dx.doi.org/10.11606/t.11.2018.tde-15032018-100208

Torgerson PR, Devleesschauwer B, Praet N, Speybroeck N, Willingham AL, Kasuga F, et al. World Health Organization Estimates of the Global and Regional Disease Burden of 11 Foodborne Parasitic Diseases, 2010: A Data Synthesis. PLoS Med. 2015 Dec;12(12):e1001920.

Cook AJ, Gilbert RE, Buffolano W, Zufferey J, Petersen E, Jenum PA, et al. Sources of toxoplasma infection in pregnant women: European multicentre case-control study. European Research Network on Congenital Toxoplasmosis. BMJ. 2000 Jul 15;321(7254):142–7.

Companhia Nacional de Abastecimento, Conab. CONAB-Acompanhamento da safra brasileira de cana-de-açúcar. v. 4 - Safra 2017/18, n.4 - Quarto levantamento, abr. de 2018 [Internet]. Acomp. safra bras. cana. Vol. 6. p. 1–58. Available from: https://www.conab.gov.br/info-agro/safras/cana

Netto CF, Others. Análise de viabilidade técnica e econômica do recolhimento de palha de cana-de-açúcar por forrageira e colheita integral. 2018; Available from: http://repositorio.unicamp.br/handle/REPOSIP/333177

De Armas R, Legaz ME, Martínez M, Medina I, Rodríguez CW, Vicente C. Composition of Some Soluble Carbohydrate Fractions from Sugarcane Juice. In: Proceedings XX Congress ISSCT. 1989. p. 625–33.

Santos NC dos, dos Santos NC. Tabela Brasileira de Composição de Alimentos (TBCA-USP): dados de flavonóides [Internet]. Available from: http://dx.doi.org/10.11606/d.89.2009.tde-21102009-164855

Martini C, Verruma-Bernardi MR. Yeast composition of sugar cane juice in relation to plant varieties and seasonality. Bioscience [Internet]. 2011; Available from: https://www.researchgate.net/profile/Sandra_Antonini/publication/267297422_YEAST_COMPOSITION_OF_SUGAR_CANE_JUICE_IN_RELATION_TO_PLANT_VARIETIES_AND_SEASONALITY_COMPOSICAO_DE_LEVEDURAS_DO_CALDO_EM_RELACAO_AS_VARIEDADES_DE_CANA_E_SAZONALIDADE/links/555b1fa708ae6943a8787bdb.pdf

Moura LBD, Cavalcante LGC, Nonato R, Júnior CM, de Rodrigues Leitão JMS, Fernanda E, et al. Avaliação microbiológica do caldo de cana comercializado em lanchonetes no Centro de Teresina-Piauí. Revista Eletrônica Acervo Saúde/Electronic Journal Collection Health ISSN. 2018;2178:2091.

Sprenger LK, Risolia LW. Condições higiênicas sanitárias da produção do caldo de cana em Curitiba, Paraná [Internet]. Vol. 22, Archives of Veterinary Science. 2017. Available from: http://dx.doi.org/10.5380/avs.v22i2.38904

Gassen GS, de Peder LD, da Silva CM. Análise da qualidade microbiológica do caldo de cana comercializado em um município da região oeste do paraná [Internet]. Vol. 9, COLLOQUIUM VITAE. 2017. p. 07–12. Available from: http://dx.doi.org/10.5747/cv.2017.v09.n3.v203

Silva SRM da, da Silva SRM, Maldonade IR, Ginani VC, Lima SA, Mendes VS, et al. Detection of intestinal parasites on field-grown strawberries in the Federal District of Brazil [Internet]. Vol. 47, Revista da Sociedade Brasileira de Medicina Tropical. 2014. p. 801–5. Available from: http://dx.doi.org/10.1590/0037-8682-0044-2014

Tchounga KS, Ajugwo AO, Nsa M, Oshoma CE, Dunga KE, Ikenazo H. Prevalence of Intestinal Parasites in Vegetables Sold in Some Local Markets in Port-Harcourt, Rivers-State, Nigeria [Internet]. Archives of Microbiology & Immunology. 2017. p. 41–9. Available from: http://dx.doi.org/10.26502/ami.9365006

Salma B, Sandie E-B, Rajae H, Antoine H, Pierre F, Loïc F, et al. Detection methods and prevalence of transmission stages of Toxoplasma gondii, Giardia duodenalis and Cryptosporidium spp. in fresh vegetables: a review. Parasitology. 2020 Jan 22;1–29.

Marafon GJ, Ribeiro MA. Os estudos agrários no âmbito da geografia oficial brasileira: o instituto brasileiro de geografia e estatística (ibge) e o periódico revista brasileira de geografia (RBG) – 1940-2005 / agrarian studies in the framework of official brazilian geography [Internet]. Vol. 0, Geo UERJ. 2017. Available from: http://dx.doi.org/10.12957/geouerj.2017.32069

De Carli (ed) GA. Parasitologia clinica: seleçao de metodos e tecnicas de laboratorio para o diagnostico das parasitoses humanas. Atheneu; 2001. 810 p.

Grumezescu AM, Butu A. Food Safety and Preservation: Modern Biological Approaches to Improving Consumer Health. Academic Press; 2018. 696 p.

Shikanai-Yasuda MA, Brisola Marcondes C, Guedes LA, Siqueira GS, Barone AA, Dias JCP, et al. Possible oral transmission of acute Chagas’ disease in Brazil [Internet]. Vol. 33, Revista do Instituto de Medicina Tropical de São Paulo. 1991. p. 351–7. Available from: http://dx.doi.org/10.1590/s0036-46651991000500003

Barbosa J de A, de Arruda Barbosa J, de Farias Guedes VH, de Moura MJA, de Arruda Ferreira Costa LG, dos Santos RL. Amostragem para determinação da atividade da redutase do nitrato em folhas de cana-de-açúcar [Internet]. 2016. Available from: http://dx.doi.org/10.31692/2526-7701.icointerpdvagro.2016.00004

Ribeiro CAF, Horii J. Potencialidades de linhagens de levedura Saccharomyces cerevisiae para a fermentação do caldo de cana. Sci Agric. 1999;56(2):255–63.

Ahmad F, Jameel AT, Kamarudin MH, Mel M. Study of growth kinetic and modeling of ethanol production by Saccharomyces cerevisae. Afr J Biotechnol. 2011;10(81):18842–6.

Lyumugabe F, Uyisenga JP, Songa EB, Thonart P. Production of traditional sorghum beer “Ikigage” using Saccharomyces cerevisae, Lactobacillus fermentum and Issatckenkia orientalis as starter cultures. Food Nutr Sci [Internet]. 2014;2014. Available from: https://www.scirp.org/html/4-2700910_43599.htm

Chispe PM, Stagg DA. Entamoeba histolytica durante el embarazo: patogenia y tratamiento. Revista Medicina. 2000;6(4):260–2.

EM International. Prevalence of intestinal parasites and differentiate entamoeba histolytica/ e.dispar/ e. moshkovskii in referred patients to the medical centers of zahedan, iran. http://www.envirobiotechjournals.com/article_abstract.php?aid=8625&iid=248&jid=4

Santos A de O, Biondi GF. Qualidade das hortaliças comercializadas no Distrito Federal. Hig alim. 2009;138–41.

Pires SM, Fischer-Walker CL, Lanata CF, Devleesschauwer B, Hall AJ, Kirk MD, et al. Aetiology-Specific Estimates of the Global and Regional Incidence and Mortality of Diarrhoeal Diseases Commonly Transmitted through Food. PLoS One. 2015 Dec 3;10(12):e0142927.

Coelho CH, Durigan M, Leal DAG, Schneider A de B, Franco RMB, Singer SM. Giardiasis as a neglected disease in Brazil: Systematic review of 20 years of publications. PLoS Negl Trop Dis. 2017 Oct;11(10):e0006005.

Gonzales I, Rivera JT, Garcia HH, Cysticercosis Working Group in Peru. Pathogenesis of Taenia solium taeniasis and cysticercosis. Parasite Immunol. 2016 Mar;38(3):136–46.

Jourdan PM, Lamberton PHL, Fenwick A, Addiss DG. Soil-transmitted helminth infections. Lancet. 2018 Jan 20;391(10117):252–65.

Pina FAN, Espinheira MJCL, de Souza FM. Análise Parasitológica de Caldos de Cana Comercializados em Feiras Livres em uma Cidade no Interior da Bahia. ID on line Rev Psicol. 2018;12(40):859–69.

Plantando saúde: resolução estabelece normas de higiene para alimentos e bebidas à base de vegetais. Rev Saúde Pública. 2005;39(5):861–3.

Brasil CCB, Silva JP, Hautrive TP. Boas práticas de manipulação e capacitação para o comércio ambulante de alimentos e bebidas. Available from: http://www.schenautomacao.com.br/ssa/envio/files/264_arqnovo.pdf

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2020-02-11

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Brandão , F., Mota, P. F. da, Monteiro, L. B. O., Shadday, L., Araújo, C., Mendes, K. G. B., & Reis, A. K. V. (2020). Parasitological analysis of sugarcane juice marketed in the Federal District. Revista De Divulgação Científica Sena Aires, 9(1), 65–76. Retrieved from https://rdcsa.emnuvens.com.br/revista/article/view/626

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Vol. 9 No. 1 (2020): REVISA

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